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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) A structural classification of the variant surface glycoproteins of the African trypanosomePLOS Neglected Tropical DiseasesSeptember 1, 2023Sara Đaković, Johan P. Zeelen, Anastasia Gkeka, Monica Chandra, Monique van Straaten, Konstantina Foti, Janet Zhong, Evi P. Vlachou, Francisco Aresta-Branco, Joseph P. Verdi, et al.were collected and incubated with 200ul FITC conjugated VSG2WT antisera (FITC conjugation kit, Abcam ab102884) (1:200) in cold HMI-9 without FBS for 10 min on ice. Cells were washed twice with cold HMI-9, resuspended

(2) A Spike-destructing human antibody effectively neutralizes Omicron-included SARS-CoV-2 variants with therapeutic efficacyPLoS pathogensJanuary 1, 2023Meng L, Zha J, Zhou B, Cao L, Jiang C, Zhu Y, Li T, Lu L, Zhang J, Yang H, et al.conjugation) His-tagged SARS-CoV-2 RBD (Cat: 40592-V08B, Sinobiological) was conjugated with FITC (Cat: ab102884) and (Cat: ab102918) Lightning-Link Conjugation Kits, respectively, according to the manufacture’s procedure

(3) Expression Analysis of Five Different Long Non-Coding Ribonucleic Acids in Nonsmall-Cell Lung Carcinoma Tumor and Tumor-Derived ExosomesDiagnostics (Basel)December 17, 2022Samaneh Talebi, Asal Jalal Abadi, Golnesa Kazemioula, Nayyerehalsadat Hosseini, Forough Taheri, Saba Pourali, Touba Mahdloo, Marzieh Rezaei, Mohammadreza Mirinezhad, Naser Ajami, et al.including CD81 and CD63. Isolated EV pellets were diluted in PBS, and incubated with FITC-(catalog: ab102884, Abcam, USA)-conjugated anti-CD63 (catalog: ab59479, Abcam, US) or anti-CD81 antibodies (catalog: ab79559,

(4) In vivo single-cell transcriptomics reveal Klebsiella pneumoniae skews lung macrophages to promote infectionEMBO molecular medicineDecember 7, 2022Dumigan A, Cappa O, Morris B, Sá Pessoa J, Calderon-Gonzalez R, Mills G, Lancaster R, Simpson D, Kissenpfennig A, Bengoechea JA.565527, Thermo Fisher), CD163 purified antibody (Ref: 155302) conjugated with FITC conjugation kit (Ref: ab102884, Abcam), CD206 FITC (Ref: 141704) according to manufacturer's instructions. Cells were incubated with

(5) Surface translocation of ACE2 and TMPRSS2 upon TLR4/7/8 activation is required for SARS-CoV-2 infection in circulating monocytesCell discoverySeptember 9, 2022Yao Y, Subedi K, Liu T, Khalasawi N, Pretto-Kernahan CD, Wotring JW, Wang J, Yin C, Jiang A, Fu C, et al.(Antibodies-online Inc., ABIN6952432, Limerick, PA) was labeled with the FITC conjugation kit—lightning-link (Abcam, ab102884). The fixed cells were then permeabilized and stained with the conjugated SARS-CoV-2 N-FITC antibody

(6) Interfacial Water in the SARS Spike Protein: Investigating the Interaction with Human ACE2 Receptor and In Vitro Uptake in A549 CellsLangmuirJuly 5, 2022Ajay Vikram Singh et al.fluorescently tagged with the fluorescein isothiocyanate (FITC) conjugation kit (Abcam Lightning-Link Cat# ab102884, Germany) were used to covalently link the dye. The gold-maleimide conjugation kit from Abcam (Cat#

(7) A broad-spectrum and highly potent human monoclonal antibody cocktail for rabies prophylaxisPLOS ONESeptember 1, 2021Pan Kyeom Kim et al.FITC-conjugated rabies virus G-protein was prepared, using an FITC conjugation kit (Abcam, Catalog No. ab102884). Labeled cells were isolated using FACS and plated onto a 96-well PCR plate with single cell. Synthesis

(8) Humanized COVID-19 decoy antibody effectively blocks viral entry and prevents SARS-CoV-2 infectionEMBO molecular medicineJanuary 11, 2021Huang KY, Lin MS, Kuo TC, Chen CL, Lin CC, Chou YC, Chao TL, Pang YH, Kao HC, Huang RS, et al.analysis tools. Flow cytometry ACE2‐Fc was conjugated with green fluorescence using a FITC Labeling Kit (ab102884, Abcam). H1975‐Spike‐overexpressing cells (2 × 105/reaction) were detached by 0.48 mM EDTA and then

(9) A Porcine Ex Vivo Lung Perfusion Model To Investigate Bacterial PathogenesismBioDecember 24, 2019Amy Dumigan, Marianne Fitzgerald, Joana Sá-Pessoa Graca Santos, Umar Hamid, Cecilia M. O’Kane, Danny F. McAuley, Jose A. Bengoecheaphycoerythrin (PE; ab102918), allophycocyanin (APC)-Cy5.5 (ab102855), fluorescein isothiocyanate (FITC; ab102884), and rhodamine (ab188286) conjugation kits. Generation of porcine bone marrow-derived macrophages

(10) Membrane expression of thymidine kinase 1 and potential clinical relevance in lung, breast, and colorectal malignanciesCancer Cell InternationalDecember 1, 2018Evita G. Weagel et al.17]. The three custom antibodies were conjugated to FITC using a conjugation kit (EasyLink, Abcam, ab102884) and stored in the dark at 4 °C. The commercially available antibody (ab91651) was conjugated to FITC

(11) Evaluation of a simple method for storage of blood samples that enables isolation of circulating tumor cells 96 h after sample collectionJournal of biological research (Thessalonike, Greece)December 1, 2017Apostolou P, Ntanovasilis DA, Papasotiriou I.Nyon, Switzerland) and Monoclonal Anti pan-Cytoceratin (SAB4700666, Sigma-Aldrich) labeled with FITC (ab102884, Abcam) according to manufacturer’s instructions. Slides were washed twice with wash buffer and finally

(12) Human Astrocyte Maturation Captured in 3D Cerebral Cortical Spheroids Derived from Pluripotent Stem CellsNeuronAugust 16, 2017Sloan SA, Darmanis S, Huber N, Khan TA, Birey F, Caneda C, Reimer R, Quake SR, Barres BA, Paşca SP.7102 Next Ultra RNA-seq library prep kit for Illumina,NEB,Cat# NEBE7530 FITC conjugation kit,Abcam,Cat# ab102884 miRNeasy Micro Kit,Qiagen,Cat# 217084 Live/Dead Viability/Cytotoxicity kit,Thermo Fisher Scientific,Cat#

(13) Biomarker analysis and clinical relevance of TK1 on the cell membrane of Burkitt's lymphoma and acute lymphoblastic leukemiaOncoTargets and therapyJanuary 1, 2017Weagel EG, Meng W, Townsend MH, Velazquez EJ, Brog RA, Boyer MW, Weber KS, Robison RA, O'Neill KL.antibodies were conjugated to fluorescein isothiocyanate (FITC) using a conjugation kit (EasyLink, ab102884; Abcam) and stored in the dark at 4°C. The custom antibodies were used for most assays, and the commercially

(14) Fibulin-1 regulates the pathogenesis of tissue remodeling in respiratory diseasesJCI InsightJune 16, 2016Gang Liu et al.Postn, Fbln1, and Fn antibodies with PE/Cy5 (clone ab102893), AMCA (clone ab102846), and FITC (clone ab102884) conjugation kits (all from Abcam) in different groups (4°C, overnight). Fluorescence signals were examined

(15) Molecular Targets of Androgen Signaling that Characterize Skeletal Muscle Recovery and RegenerationNuclear Receptor SignalingOctober 7, 2015James G. MacKrell, Benjamin C. Yaden, Heather Bullock, Keyue Chen, Pamela Shetler, Henry U. Bryant, Venkatesh Krishnananti-Pax7 antibody (MAB1675; R&D systems) was conjugated together with FITC (FITC Conjugation kit; Abcam ab102884). For mouse muscle IHC, the anti-Pax7 primary antibody was purchased from Abcam (Ab34360). The non-steroidal

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FITC Conjugation Kit - Lightning-Link from Abcam

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FITC Conjugation Kit - Lightning-Link from Abcam

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